Fig. 3. Twist protein is normal in most embryos derived from egg chambers with dorsal Ras clones. (A-D) Confocal projections (black/white-inverted) of anti-Twist fluorescence in cellularizing embryos hand-picked based upon dorsal appendage phenotype. Anterior is to the left in all panels. VM, ventral midline; DM, dorsal midline. (A) Twist protein in an embryo derived from a control clone egg chamber. 21 of 26 embryos derived from egg chambers with dorsal Ras clones were indistinguishable from controls. (B-D) Three examples of embryos derived from egg chambers with dorsal Ras clones that did display ectopic Twist protein (arrowheads), which always contacted the normal ventral Twist domain.