Fig. 10. Northern blot analysis of Aqp5 and Foxp2 gene expression in the lungs of SP-C/G6en mice. Total RNA (10 µg) from E19.5 wild-type (lanes 1 and 2) and SP-C/G6en (lanes 3 and 4) dissected lung tissue was analyzed by northern blot analysis using radiolabeled probes specific for Aqp5, Foxp2, and the engrailed repression domain (to detect transgene expression). Bottom panel represents the ethidium bromide-stained gel before transfer showing equal loading of RNA in all the lanes. All RNAs represent individual and separate F0 E19.5 wild-type or SP-C/G6en transgenic littermates.