Fig. 5. Effects of misexpression of BR on cuticle gene expression during adult development. (A) Northern analysis of mRNAs for BR-Z1, the pupal cuticle protein Edg78E, and the adult cuticle protein Acp65A in the transgenic fly line (w¹¹¹⁸; 708-14; 708-1) containing four copies of the BR-Z1 isoform variant (TNT-Q¹-Q²-Z1) under a heat shock promoter. Pupae were harvested at various times without heat shock or after a 30 minute heat shock starting at 48 hours APF. (B) Misexpression of each of the BR isoforms has different effects on cuticle gene expression. Each of the BR isoforms was heat-induced as in A at the designated time between 36 and 60 hours APF, then the tissue was collected at 66 hours APF for the pupal cuticle genes and at 72 hours for the adult cuticle gene (the time of its peak expression in both regions; see A). We used transgenic lines with four copies of Z1 as in A and of each of the other Z isoforms as described in the Materials and Methods. (C) Effects of misexpression of BR-Z1 at 64 hours APF on the expression of cuticle genes at 72 hours APF. In all cases, puparia were maintained at 25°C before and after the heat shock. RNA analysis was carried out as in Fig. 3.