Fig. 3. Neurofilament-expressing cells in the CMZ are newly generated and express markers of differentiated neurons. (D-F) Vertical sections of (A-C,G-I,M-O) the retinal margin and (D-F,J-L) the central retina were labeled with antibodies to (A; red) the 160 kDa subunit of neurofilament, (D,G,J,M; red) 145 kDa subunit of neurofilament, (B; green) BrdU, (E,H; green) Hu, and (K,N; green) Islet1. C,F,I,L,O are merged images. Tissues were obtained from eyes that were treated with three consecutive daily injections of insulin and FGF2. The small arrows indicate double-labeled cells, and the large arrowhead in J-L indicates a neurofilament-positive ganglion cell that does not express Islet1. Scale bars: in C, 50 µm in A-C; in O, 50 µm in D-O. NF, neurofilament; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.