Fig. 6. Insulin and FGF2 induce the production of neurofilament-expressing ganglion cells at the retinal margin. Whole-mount preparations of the retinal margin were obtained from eyes 4 days after three consecutive daily injections of insulin and FGF2. Tissues were labeled with antibodies to (A,C,D,F; green) BrdU and (B,C,E,F; red) neurofilament. Large arrows indicate the retinal margin and small arrows indicate double-labeled cells. Scale bar: 50 µm.