Fig. 7. Cath5 is expressed at the retinal margin of eyes treated with insulin and FGF2. Vertical sections of the retinal margin were labeled for Cath5 transcripts. In situ hybridization was used to detect Cath5 mRNA in tissues treated with (B) insulin alone or (A,C) insulin and FGF2. Tissues were processed for in situ hybridization 24 hours after the final dose of three consecutive daily injections of growth factor. Large arrows in B,C indicate the retinal margin and the small arrows indicate Cath5-expressing cells. The boxed area in C is increased 2.5-fold below to better demonstrate Cath5-expressing cells. ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar: 50 µm. (D) Histogram demonstrating the numbers of Cath5-expressing cells observed at the retinal margin of eyes treated with insulin alone, FGF2 alone, and a combination of insulin and FGF2.