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Fig. 3. Jun expression in the sclerotome and notochord is required for skeletogenesis. (A,B) Skeletal X-ray analysis of adult human Col2a1;Jun{Delta}/{Delta} (B) and Junf/f control (A) mice. Arrowheads indicate scoliosis. (C,D) Alcian Blue/Alizarin Red skeletal preparations of the thoraxic region of adult Col2a1;Jun{Delta}/{Delta} (D) and Junf/f control (C) mice. (E-H) mRNA in situ analysis of E14.5 Col2a1;Jun{Delta}/{Delta} (F,H) and Junf/f control foetuses (E,G) using a Col2a1 (E,F) and a collagen X (G,H) antisense probe.