Fig. 2. The glial cell undergoes apoptosis. (A,B) Deletion of the pro-apoptotic genes rpr, grim and hid in H99-deficient clones blocked fragmentation of the glial cell. Clones were detected due to their lack of GFP staining (green); their limits are shown with a white line. Sensory organ cells were identified by anti-Cut immunoreactivity (also in green). Glial cells were immunostained with anti-Repo antibodies (red). Repo-labelled glial cells are observed in clusters inside H99 deficient clones at 24 hours APF (arrowheads in A). Note that, outside the clone, in the homozygous wild-type twin clone (GFP positive cells on the left of the figure), no glial cell was observed. (B) At 30 hours APF, some sensory organs in H99-deficient clones were associated with a glial cell (arrowheads). Note also organs in which the glial cell was not present (asterisk). (C,D) Overexpression of p35 represses glial cell fragmentation. neu^P72 UAS-H2B::YFP UAS-p35pupae dissected at 24 hours APF (C) and 30 hours APF (D). Sensory cells are in green; Repo immunostaining is in red. Note that glial cells are still present at 24 hours APF (arrowheads in C) and have disappeared at 30 hours APF (D). Note also that no cell fragments are observed. Anterior is upwards and the view is horizontal.