Fig. 4. Ectopic glial cells were frequently associated with growing axons. Growing axons from neu^P72 UAS-H2B::YFP UAS-p35 sensory clusters were immunostained with 22C10 antibodies (shown in green). YFP expressing sensory cells are depicted in red. Glial cells (arrowheads) were maintained alive after overexpression of p35. In A-C, typical positions of the ectopic glial cell are shown relative to the growing axon at 24 hours APF. The glial cell was associated with the growth cone (A), standing along the axon (B) or near the sensory cluster even when the axon was well developed (C). Clusters on the first row of microchaete are shown. Clusters in B and C are from the same notum. Another example of growth cone-associated glial cell is shown on the second row of microchates (D). Anterior is upwards and the view is horizontal.