Fig. 5. Ectopic glial cells promote precocious axogenesis. (A,B) Camera lucida drawing of nota stained with 22C10 antibodies at different times of development. In each case, the entire region between the two dorsocentral macrochaetes is depicted. (A) Wild-type nota. At 24 hours APF, only axons from macrochaetes were present. Axogenesis of microchaetes starts at around 25 hours APF. (B) Nota from neu^P72 UAS-H2B::YFP UAS-p35. Microchaete axogenesis initiates at about 23:30 hours APF. Note that in nota older than 27 hours APF, no major difference regarding the orientation of axonal processes could be discerned. (C) H99-deficient clone stained with 22C10 antibody (shown in green on the bottom overlay image). The clone was detected because of its lack of GFP staining (shown in red on the bottom overlay image), its medial limit is shown with a white line. Each channel is shown individually (22C10 top left and GFP top right). The midline is associated with a dotted 22C10 immunoreactivity of unknown nature. Note that axon projections are more developed in sensory organs inside the clone (arrowheads) than the counterpart axons in the contra-lateral region (H99 heterozygous). Limits of clones are indicated with a white line. Anterior is upwards and the view is horizontal.