Fig. 2. Comparison of the expression domains of Six3, Six6 and Gro/Tle genes in medaka embryos. Whole-mount in situ hybridisations at different developmental stages as indicated at the top of each colum. All embryos are dorsalview, anterior to the left. Embryos were hybridised with probes to Six3 (A-C), Tle1 (D-F), Tle3 (G-I), Tle4 (J-L), Aes (M-O) and Six6 (P-R). Note how Tle1 and Aes are expressed in the eye field from early stages. Arrowhead in H indicates the lens vesicle; arrowhead in K, the optic stalk; arrows in O, Q and R, the ventral diencephalon. ov, optic vesicle; ey, eye; mb, midbrain; hb, hindbrain. Scale bars 0.1 mm.