Fig. 3. TLE1 over-expression enlarges the eye field in medaka embryos. Dorsal (A-D,G,H) and ventral (E,F) views (anterior to the left) of TLE1-injected embryos. (A) Over-expression of TLE1 (inset shows expression of the co-injected GFP mRNA) causes a visible enlargement of the optic vesicles and bulging of the midbrain (arrowhead). Whole-mount in situ hybridisations demonstrate that expression of Six3 (B) and Rx2 (D) are expanded (arrows) compared to control embryos (C, and Fig. 2B). Note that TLE1 injections lead to the expansion of the posterior domain of Pax6 and to the appearance of ectopic Pax6 expression (arrowhead in F), as compared to controls (E). Otx2 expression was similar to that of controls (G,H). ey, eye; mb, midbrain; hb, hindbrain; wt, wild type. Scale bar: 0.1 mm.