Fig. 5. AES over-expression reduces the eye size in medaka embryos. Dorsal (except E, ventral) views of embryos at stage 24 (A,C,G,H,I), stage 32 (B,D,E) and stage 20 (F) injected with AES. Anterior is to the left. Dotted white lines indicate the extent of the eye domains. Embryos show a unilateral (A) or bilateral (D) loss of the eye(s). (A inset) Expression of the co-injected GFP mRNA. (B) The same embryos as in A but at a later stage of development, showing that the failure of eye formation (arrowhead) is permanent. Whole-mount in situ hybridisations demonstrate that the reduction or absence of the eye(s) is accompanied by a decrease of the expression domain of Six3 (arrows in C,E) and Rx2 (arrows in F,G). Apart from the reduced domain of the affected eye (arrow in H), Pax6 (H) and Otx2 (I) expression domains were similar to those of wild-type embryos. Occasionally, ectopic expression of Otx2 was observed in the hindbrain (arrow in I). mb, midbrain. Scale bars: 0.1 mm.