Fig. 5. Adult phenotypes (A,B,G) and gene expression patterns caused by the ectopic expression of vg in clones located in the leg (C-F) and eye (H,I) imaginal discs. Adult clones are labelled with f and delimited by a broken red line. In the imaginal discs, vg clones are associated with GFP. Scale bars: 0.1 mm. (A) Clones of vg ectopic expression include `mixed' cuticular differentiation patterns in the leg, with leg chaetae mixed in a `salt and pepper' distribution with wing blade trichomes. In the inset, compare trichomes of the leg (black arrowhead) with wing blade trichomes (red arrowhead). The age of clone initiation is 36±12 hours AEL. (B) Proximalisation phenotypes of the leg without cuticular transformation induced by vg ectopic expression in clones. In distal tarsal segments of the leg, typical chaetes are associated with bracts (black arrowhead in the inset), whereas in vg clones chaeates are not associated with bracts (red arrowhead in the inset). Notice the non-autonomous size reduction in distal tarsal segments of the leg. The age of clone initiation is 36±12 hours AEL. (C) Clones of vg ectopic expression (green) autonomously reduce endogenous wg expression (red; white arrowhead). The age of clone initiation is 60±12 hours AEL. (D) Clones of vg ectopic expression (green) may activate bs (red) expression (white arrowhead) in a subset of cells within the clone. Notice that only those clones situated in ventral leg territories induce bs expression. The age of clone initiation is 60±12 hours AEL. (E,F) Clones of vg ectopic expression (green) in ventral territories of the leg show autonomous expression of the ap-lacZ reporter (red) (E), whereas it is repressed in dorsal territories (F). Notice that ap expression is not detected in all cells of the clone, similar to D. The age of clone initiation is 60±12 hours AEL. (G) Outgrowth with wing blade trichomes (indicated by red arrowhead) induced by vg clone. In the inset, trichomes with wing blade characteristics (red arrowhead) are compared with surrounded ommatidial differentiation (black arrowhead). The age of clone initiation is 36±12 hours AEL. (H) Clones of ectopic vg expression (green) autonomously reduce the expression of wg (red; white arrowhead). The age of clone initiation is 60±12 hours AEL. (I) In the eye, clones of vg ectopic expression (green) autonomously induce the expression of nub (white arrowhead). Only those clones induced in territories with high levels of Wg express nub. The age of clone initiation is 60±12 hours AEL.