Fig. 6. Cuticular phenotypes (A) and imaginal disc expression pattern (B-D) caused by clonal overexpression of wg in the wing imaginal disc. Adult clones are labelled with f and delimited by a broken red line. In the imaginal discs, wg clones are associated with GFP. Scale bars: 0.1 mm. (A) Clones of wg overexpression in the notum show the typical differentiation of this territory. In the inset notice that trichomes are equal inside (red arrowhead) and outside the clone (black arrowhead). The age of clone initiation is 36±12 hours AEL. (B) Clones of wg overexpression (green) in the wing blade autonomously and non-autonomously induce the expression of vg (red), whereas those clones outside the wing blade do not express vg (white arrowhead). The age of clone initiation is 60±12 hours AEL. (C) Clones of wg overexpression (green) in the wing imaginal disc autonomous and non-autonomously induce the expression of nub (red; white arrowhead). Notice that non-autonomous expression of nub is detected only in the closest cells surrounding the clone. The age of clone initiation is 60±12 hours AEL. (D) Clones of wg overexpression (green) show homogenous and high levels of Wg (red). The age of clone initiation is 60±12 hours AEL.