Fig. 8. Adult phenotypes (A,B) and imaginal disc expression patterns (C,D) caused by the ectopic clonal co-expression of wg and vg in the leg (A,C) and eye (B,E) imaginal discs. Adult clones are labelled with f and delimited by a broken red line. In the imaginal discs, clones are associated with GFP. Age of clone initiation is 60±12 hours AEL. Scale bars: 0.1 mm. (A) wg-vg clones induce a `mixed' cuticular differentiation pattern in the leg. Notice that in the outgrowth chaetae characteristic of the leg appear mixed with wing blade trichomes in a `salt and pepper' distribution. The inset shows the differences between wing blade trichomes of the clone (red arrowhead) and leg trichomes outside the clone (black arrowhead). (B) In the eye, all cells of the wg-vg clones autonomously differentiate into sensory elements of the wing margin. (C) In the leg, wg-vg clones activate the expression of wing blade genes such as bs (red), only in a subset of cells within the clones (arrowhead). Notice that the expression of bs is not detected in all the clones. (D) In the eye, wg-vg clones (GFP) autonomously activate the expression of nub (red) in all positions.