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Fig. 1. Gfi1 wild-type expression pattern. In situ hybridization using a specific antisense probe derived from the 3' UTR of Gfi1 and immunohistochemistry using a Gfi1-specific antibody. For in situ, positive cells are purple, and for immunohistochemistry positive cells are brown and counterstained with Hematoxylin (purple). (A) Sagittal section of E12.5 embryo. Areas of high expression of Gfi1 mRNA are denoted and include the developing brain (FB, forebrain; HB, hindbrain), optic epithelia (Op), dorsal root ganglia (DRG) and gut epithelia (Gut). In addition, Gfi1 mRNA is expressed in the developing otic vesicle (OV) and several ganglia including the trigeminal (V) and vestibulo-cochlear ganglia (VIII) (see Fig. 2A for enlarged view of ear and associated ganglia). (B) Sagittal section of E18.5 retina showing Gfi1 expression primarily in the retinal ganglion cell layer. (C) Sagittal section of E16.5 retina showing immunohistochemistry of Gfi1 expression in specific cells denoted with arrows that are likely to be retinal ganglion cells. (D) Gfi1 expression in E16.5 section through the upper lip and mouth area where the whiskers develop. Positive cells around the shaft of the whiskers are thought to be the Merkel cells. (E) Gfi1 expression in E15.5 section through the skin. Positive cells are located under the touch domes and correspond in size and position to the Merkel cells. (F) E18.5 section through the upper lip and mouth area where the whiskers develop. Immunohistochemically positive cells (brown cells) around the shaft of the whiskers denoted by arrows are thought to be the Merkel cells. (G) Gfi1 expression in E16.5 section of the olfactory epithelia. (H) Gfi1 expression in E18.5 section of the tongue and its dorsal epithelium where taste papilla develop. (I) Gfi1 expression in E15.5 section of the developing lung. Clusters of cells as well as individual cells express Gfi1. (J) Gfi1 protein expression in E16.5 section of the developing lung. Clusters of cells denoted by arrows express Gfi1. (K) Gfi1 mRNA expression in E15.5 section of the developing thymus.