Fig. 2. (A) Promoter/reporter constructs generated by fusing the 5' region of the human TAX1 gene to either a lacZ reporter or the F3/contactin cDNA. (B) Expression of TAG-1 protein and lacZ reporter in the cerebellar cortex of TAG/lacZ transgenic mice at postnatal day 8. (a-c) TAG-1 immunostaining (a) and X-gal staining (c) of sequential sagittal sections obtained at the level of the vermis. In b a double TAG-1/lacZ staining is shown. In a, the lobules are indicated by roman numerals. Scale bars: 200 µm. (d-f) Expression of the TAG-1 protein (d) and of the TAX-1/lacZ transgene (f) in lobules V-VI. Double TAG-1/ lacZ staining is reported in e. Scale bars: 100 µm. (g-i) TAG-1 (g) and lacZ (i) expression in the cerebellar cortex of lobules VIII-IX. h shows a double TAG-1/X-gal staining. Scale bars: 40 µm. Sections c-f-i are counterstained with Fast Red.