Fig. 3. Expression profile of the TAX-1/F3 transgene. (A,B) Expression of the TAX-1/F3 fusion mRNA in transgenic mice cerebellum throughout development, demonstrated by RT/PCR. (A) Amplification scheme. (A) TAX1 exon 1-derived forward primer (nt 146-169) (Kozlov et al., 1995) was used with an F3/contactin exon VII-derived reverse primer (nt 855-834) (Gennarini et al., 1989). (B) A 716 nt band could be detected throughout development. An oligonucleotide pair derived from the mouse actin gene generated a 140 nt band we used as an internal control. (C) Expression profile of F3/contactin in the cerebellar cortex of wild-type (a,c) and TAG/F3 (b,d) mice at postnatal day 4. Scale bars: 40 µm.