Fig. 4. Cerebellar phenotype of developing wild-type and TAG/F3 mice shown in sagittal sections stained with Toluidine Blue. (A) Low magnification images of P3 (a,b), P8 (c,d), P11 (e,f) and P16 (g,h) cerebella. In sections from wild-type mice the lobules are indicated by roman numerals. Scale bars: 200 µm. (B) Differences in layer morphology between wild-type (i,k,m,o) and TAG/F3 (j,l,n,p) mice, shown in lobule VIII at P3 (i,j), P8 (k,l), P11 (m,n), P16 (o,p). The different cortical layers are indicated on the right side. EGL, external germinal layer; ML, molecular layer; PCL, Purkinje cells layer; IGL; inner granular layer. Scale bars: (i-n) 40 µm; (o,p) 100 µm. (C) Morphometric analysis of TAG/F3 and wild-type mice cerebella. The overall surface areas of the cerebellar sections, the EGL, IGL and ML, from P0 to P30, were expressed as the ratio of their values in transgenic versus wild-type mice. Asterisks refer to statistical significance of the differences estimated in the absolute values of the different parameters.