Fig. 5. (A) Analysis of precursor granule cell proliferation in the developing cerebellum of wild-type and TAG/F3 mice. 5-bromo-2'-deoxyuridine (BrdU) incorporation in lobules VIII-IX of developing cerebellar cortex from wild-type (a,c,e,g,i) and TAG/F3 (b,d,f,h,j) mice at P0 (a-b), P3 (c-d), P6 (e-f), P8 (g-h) and P11 (i-j). Scale bars: 40 µm. (B) Analysis of cell proliferation in primary cultures from postnatal day 3 cerebellum. BrdU incorporation is shown in primary cerebellar cultures from wild type (a,b) or TAG/F3 (c,d) mice labelled immediately after plating (T0) or 16 hours later (T16). Scale bars: 40 µm. In C the data are shown in graphic format. (D) Epifluorescent labelling of P8 cerebellar cortex from wild-type mice with an anti-proliferating cell nuclear antigen (PCNA) monoclonal antibody (a) and a rabbit anti-TAG-1 serum (b). (c) Confocal optical section of the same field as a and b, showing PCNA (red) and TAG-1 (green) expression. (d) Confocal optical section of P8 cerebellar cortex from wild-type mice labelled with polyclonal antibodies to phosphohistones H1 and H3 (red) and monoclonal anti-TAG-1 antibodies (green). Note that phosphohistones H1 and H3 label mitotic cells most strongly, while other phases are marked by weaker phosphohistone H1 expression alone (Lu et al., 1994); this image was contrast-enhanced to emphasise cells in mitosis thus de-emphasising labelling in other phases of the cycle. The pial surface is marked by a dashed line. Scale bars: 20 µm.