Fig. 3. DPY-22::GFP is expressed in the nuclei of vulval cells, and is mislocalized by the dpy-22(sy665) mutation. Left-hand images are Nomarski, and right-hand images are fluorescence. Scale bars: 20 µm. Arrows indicate some of the vulval cells and arrowheads indicate the anchor cells. (A-F) dpy-22(sy622) hermaphrodites rescued with a dpy-22::gfp transgene that encodes a fusion of full-length wild-type DPY-22 to GFP. (A,B) Two-cell stage at the onset of vulval fate specification. (C,D) Four-cell stage of vulval development. (E,F) Eight-cell stage of vulval development. (G,H) dpy-22(sy622) hermaphrodites rescued with a dpy-22 2548::gfp transgene, which encodes a fusion of the first 2548 amino acids of DPY-22 to GFP. (I,J) pha-1(e2123ts)-rescued animals carrying a dpy-22 2141::gfp transgene, which encodes a fusion of the first 2141 amino acids of DPY-22 to GFP. Wild-type DPY-22 and DPY-22 truncated after amino acid 2548 direct GFP to the nucleus, but not DPY-22 truncated after amino acid 2141.