Fig. 3. Western blot analysis of Ed and Nrg protein expressed by transfected S2 cell lines. Shown is a western blot analysis of induced, native (lane 1) and transfected S2 cells. Each lane contains the total protein from 2.5x10⁵ cells. Lane 2 represents cells that were transfected with a construct encoding the neuronal isoform of Nrg, whereas lane 3 contains cells that were transfected with a HA epitope-tagged version of Drosophila Ed. Blot A was incubated with the anti-Nrg monoclonal antibody BP-104 and blot B with the HA.11 monoclonal antibody, respectively.