Fig. 6. Co-immunoprecipitation of Ed and Neuroglian protein from mixed S2 cell aggregates. For this co-immunoprecipitation experiments S2 cells expressing Ed were mixed and co-aggregated with either S2 cells expressing Nrg^GPI (lanes 1 and 3) or native S2 cells (lane 2). Soluble proteins were immunoprecipitated using the anti-Nrg monoclonal antibody 1B7 (lanes 1 and 2) or a control monoclonal antibody (lane 3). The western blot shown in this figure was analyzed for the presence of Ed protein using the HA.11 monoclonal antibody.