Fig. 2. Sections of Hoxb13 mutant ventral prostates. (A,B) Hematoxylin and Eosin stained sections, and (C,D) high magnification X-gal and eosin stained sections of heterozygous (A,C) and mutant (B,D) ventral prostates. There is a lack of secretory proteins within the ducts of the homozygous mutant ventral prostates (B) when compared to heterozygotes (A, black arrows). X-gal staining reveals strong expression of the Hoxb13lacZ reporter in the luminal epithelium (C,D) Note the tall columnar epithelium in heterozygotes compared with the simple cuboidal epithelium in homozygous mutants (compare C and D, white arrowheads). Scale bars: 25 µm (in B for A,B; in D, for C,D).