Fig. 3. FRL-1MO specifically inhibited the translation of FRL-1. 10 ng of
FRL-1-6myc mRNA, which codes for FRL-1 tagged with six c-myc-epitopes
was injected with or without 40 ng of FRL-1MO or FRL-1-4misMO into oocytes. 10
ng of FRL-1
5'UTR-6myc lacking the target sequence of
FRL-1MO, was also injected with or without 40 ng of FRL-1MO. Lysates with
uninjected controls were used for western blotting. FRL-1 proteins with the
6myc-epitope tag were detected by anti-c-myc antibody. The translation of
FRL-1 was inhibited by FRL-1MO but not by FRL-1-4misMO, whereas that
of FRL-15'UTR was not inhibited by FRL-1MO.