Fig. 6. FRL-1MO inhibits the expression of neural markers without inhibition of mesodermal and endodermal markers in Xenopus embryos. 10 ng of FRL-1MO injected into each animal or vegetal blastomere of 8-cell-stage embryos or FRL-1-4misMO injected into each animal blastomere of 8-cell-stage embryos, which were cultured until stage 31 for RT-PCR. FRL-1MO vegetally injected and FRL-1-4misMO animally injected did not affect the expression of any marker when compared with controls (uninjected), whereas FRL-1MO animally injected suppressed the expression of neural markers N-CAM, otx2, ß-crystallin and N-tubulin. The suppression of neural markers was rescued by 20 pg of FRL-15'UTR.