Fig. 8. FRL-1MO inhibits the induction of neural markers by chordin in animal caps. 100 pg of chordin was injected into the animal pole of 4-cell-stage embryos with 40 ng of FRL-1-4misMO (lane 3), FRL-1MO (lane 4), FRL-1MO and 500 pg of FRL-1 (lane 5), or without MO (lane 2). The animal caps were dissected at stage 9 and harvested at stage 22 for RT-PCR. chordin induces the expression of N-CAM, otx2 and N-tubulin without the induction of mesodermal markers ms-actin and collagen type II, which was inhibited by FRL-1MO but not by FRL-1-4misMO. The suppression of neural markers caused by FRL-1MO was rescued by the co-injection of FRL-15'UTR.