Fig. 5. Chick and rodent oligodendrocyte precursors express netrin receptors. (A-D) Pan-purified O4-positive chick oligodendrocyte precursors. (A) Oligodendrocyte precursors from E10 or E12 chick spinal cords showed alternatively spliced forms (427 and 266 bp) of neogenin by RT-PCR. (B) The majority of O4-positive purified oligodendrocyte precursors (>90%) were labeled with anti-neogenin antibody, showing punctate labeling on the cell processes and brighter labeling over the cell body (C). A similar proportion of O4-positive cells were labeled with anti-DCC antibody (D). (E-H) Pan-purified A2B5-positive rat spinal cord oligodendrocyte precursors. (E) RT-PCR analyses reveal bands with sizes correlating with UNC5h1 (643 bp), UNC5h2 (446 bp) and DCC (498 bp). The amplified DCC product was further confirmed by predicted internal restriction enzyme site (DCC/cut). Most A2B5-positive (G) rat oligodendrocyte precursors were double labeled with anti-DCC antibody (H). (F) Phase contrast view. Scale bar: 20 µm.