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Fig. 2. Marker gene expression in the interrenal primordium. In situ hyridization showing expression of scc (A), 3ß-HSD (B) and ff1b (C) in the region ventral to the third somite and wt1 (D) expression in a wider region ventral to the second and third somites at 36 hpf. (E-J) Co-expression of ff1b and scc at 30 (E-G) and 33 hpf (H-J). Green indicates scc expression, red indicates ff1b expression and yellow indicates the merged signal of scc and ff1b.