Fig. 6. Knockdown of wt1 by morpholino affects early development of both the pronephros and interrenal glands. The pronephric and interrenal primordia of wild-type (A,D,G) and wt1 morphants (mo; C,F,I) are labeled with wt1 (black) and ff1b (red) (ventral view). (B,E,H) The pronephric primordia of ff1a morphants are labeled with wt1 (brown). (A-C) 24 hpf, (D-F) 36 hpf, (G-I) 2 dpf. The pronephric primordia in C are more spread out than in A and B. The pronephric primordia in F,I are smaller and unfused. The sizes of interrenal primordia and ff1b expression in wt1 morphants (C,F,I) are also reduced.