Fig. 4. The expression pattern of spr-3. (A) The stage-specific expression of the spr-3 transcript with ama-1 shown as a control; E, eggs; L1-L4, first to fourth larval stages; A, adult. (B,C) An spr-3::EGFP promoter fusion is expressed broadly throughout the animal. spr-3 is expressed uniformly in eggs (B) and very broadly in the adult (C) with strong expression in the pharynx. (D,E) Expression of a GST-Myc tagged spr-3 Baculovirus construct expressed in insect Sf9 cells and detected with a fluorescein-isothiocyanate coupled goat-anti-mouse secondary antibody. (D) Fluorescent and (E) propidium iodide staining of the same cells.