Fig. 7. Mutations in spr-3 and spr-4 de-repress the expression of the hop-1 message at the L1 stage. Expression of the hop-1 message with ama-1 loading control in the L1 stage for N2 (wild type), sel-12(ar171), four spr-5 alleles (by101, by119, by128, by139), one spr-4 allele (by130) and three spr-3 alleles (by108, by135, by136). All Spr mutants are in a sel-12(ar171) background. Fold expression of hop-1 compared with N2 is given, after correction for equal loading. The first six lanes are reproduced, with permission, from Eimer et al. (Eimer et al., 2002b). Preliminary experiments with 5 µg/lane RNA gave qualitatively similar results. See Fig. S1 at http://dev.biologists.org/supplemental/ for additional spr-3 alleles.