Fig. 5. Both Xgly4Mo and wild-type overexpression inhibit convergent extension movements. (A) Activin (0.25 pg)-induced elongation of AC explants (top right) mimics the convergent extension movements seen during gastrulation. This elongation was inhibited by Xgly4Mo (41 ng; middle left). The inhibition was rescued by a low dose (25 pg) injection of Xgly4 (middle right). A higher dose (500 pg) of Xgly4 inhibited activin-induced elongation (bottom left), but a low dose (25 pg) did not (bottom right). (B) Expression levels of Xbra, Xwnt11 and Histone (control) were analyzed by RT-PCR assay. The expression levels of Xbra and Xwnt11 were not affected in any of the explants shown in A.