Fig. 6. (A) Xgly4 enhances the Xwnt11-induced convergent extension movements. (B) Severity of affected embryos. The phenotypes were graded according to the index of severity from 0 (normal) to 3 (spina bifida). Number of each grade was indicated as percentage. Although co-injection of Xgly4 mRNA at a low dose (0.5 pg) enhanced the defective gastrulation movements caused by injecting Xwnt11 mRNA (1 ng), co-injection of Xgly4 mRNA at a high dose (25 pg) rescued these defects. This indicates that the Xgly4 protein acts as positive modulator, but inhibits Xwnt11 signaling at a high dose, just as Knypek does in silberblick (zebrafish wnt11) signaling (Topczewski et al., 2001).