Fig. 6. (A-C) Phylogeny of the conserved domains of the achaete-scute, Delta and Notch homologues. The trees were constructed using the neighbour-joining algorithm (see Materials and Methods for further details). The numbers at the nodes are the bootstrap values given in percent (1000 replicates). Nodes without numbers have bootstrap values below 50%. The numbers below the branches are the branch lengths. (A) The tree was constructed from an alignment of the bHLH domains of nine insect, five vertebrate, two spider and two myriapod sequences. Both myriapod homologues group outside the insect genes together with the spider and vertebrate homologues. (B) The tree was created from an alignment of the DSL domains and the adjacent highly conserved EGF-repeats 1 and 2 from two insect species, five vertebrate, two myriapod, an ascidian and the two spider sequences. The insects and the vertebrates form two clear groups, while the myriapods group with the spider sequences. (C) The tree was constructed from an alignment of the obtained GmNotch sequence (5' region up to EGF-repeat 12) with the same region of four vertebrate and three invertebrate Notch homologues. The myriapod sequence groups with the chelicerate Boophilus microplus, while the spider homologue forms a group with the vertebrates. The node joining the chelicerates with the vertebrates and the myriapod has a high bootstrap support (100%). Genes: L-sc, lethal of scute; Sc, scute; Ac, achaete; Ash, achaete-scute homologue; Scal, scalloped wings. Species: Ag, Anopheles gambiae; As, Archispirostreptus sp.; Bm, Boophilus microplus; Cc, Ceratitis capitata; Cs, Cupiennius salei; Csa, Ciona savigny; Cv, Calliphora vicina; Dm, Drosophila melanogaster; Dr, Danio rerio; Gg, Gallus gallus; Gm, Glomeris marginata; Hs, Homo sapiens; Jc, Junonia coenia; Lc, Lucilia cuprina; Mm, Mus musculus; Tc, Tribolium castaneum; Xl, Xenopus laevis.