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Fig. 3. Carotenoid and retinoid contents of zebrafish eggs and larval eyes. (A) Retinoid composition of the zebrafish eggs. A HPLC profile at 360 nm of an extract isolated from 180 zebrafish eggs separated on HPLC system 1. (B) Carotenoid composition of zebrafish eggs. A HPLC-profile at 450 nm of an extract isolated from 165 eggs separated on HPLC system 2. (C) Carotenoid and retinoid contents of zebrafish eggs and larval eyes (4 dpf). The values give the means of at least two independent experiments. The individual retinoids and carotenoids were analyzed and quantified on HPLC systems 1 or 2, respectively. Molar amounts were calculated by equilibration of the HPLC systems with defined amounts of authentic reference substances.