Fig. 4. Morphological phenotype of bcox morphants. (A-D) Lateral views of 4 dpf bcox morphants raised from eggs injected with 1.5 ng (B), 9 ng (C) and 18 ng (D) bcox-MO compared with a non-treated sibling (A). The bcox-MO treatment provoked malformations, i.e. a curved neck, smaller eyes and an edematically enlarged pericardial region (see arrows) occurred dose dependently in repeated independent experiments. (E,F) The pigmentation of bcox morphants (F) is altered when compared with controls (E). As indicated by arrows, the iridophores are lacking. (G) Western blot analysis of Bcox protein levels in protein extracts derived from 2-day-old bcox- MO-treated embryos and wild-type siblings.