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Fig. 3. GFR{alpha}1 and GFR{alpha}2 expression in the gut. (A,B) E14 gut expresses GFR{alpha}1 (red arrows, FITC) but not GFR{alpha}2 (FITC). Yellow arrows (B) show PGP9.5-expressing neural crest cells in the gut (Cy3 secondary), but there is no detectable GFR{alpha}2 staining. Newborn (C,D) and adult (E,F) small bowels express GFR{alpha}1 (FITC) and GFR{alpha}2 (FITC), but GFR{alpha}2 is much more easily detected in adult (F) than in newborn mouse gut. Scale bar: 100 µm.