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Fig. 2. Knockdown of Dlx3b, Dlx4b and Sox9a functions mimics the Dfb380 mutant ear phenotype. (A,D,G) Otic vesicles of wild-type embryos express pax2a, fn1 and cldna. (B,E,H) A few cells in Dfb380 mutants form a small epithelial ball and express the same markers (83/196 Dfb380 mutants expressed pax2a, 11/17 expressed fn1 and 10/20 expressed cldna). (C,F,I) Injection of a mixture of dlx3b-MO, dlx4b-MO and sox9a-MO produces a similar, small ball of cells that express the same markers (24/56 expressed pax2a, 8/20 expressed fn1 and 2/7 expressed cldna). Developmental stage: Prim-5 (24 h). Side views, anterior towards the left, dorsal towards the top. Scale bar: 25 µm.