Fig. 4. Deficient cumulus mucification in Tnfip6/
females. (A-C) Hematoxylin stained preovulatory follicles in the three
Tnfip6 genotypes 48 hours after the PMSG treatment (before hCG
injection). (D-F) Hematoxylin stained preovulatory follicles 10 hours after
induction of ovulation by hCG injection. (G-L) Localization of hyaluronan
(green) in preovulatory follicles 10 hours after hCG injection. Low (G-I) and
high (J-L) power magnifications are shown. White arrowheads in L point to cell
surface hyaluronan. Nuclei are stained blue by DAPI. Scale bars: 100 µm
(A-I) and 50 µm (J-L).