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Fig. 6. Impaired expansion of Tnfip6–/– COCs in vitro. (A-I) Compact COCs (48 hours after PMSG injection) from the three Tnfip6 genotypes were cultured in the absence (A-C) or in the presence of either 3 ng/ml EGF (D-F) or 1 mM dbcAMP (G-I). (J-K) Expansion of Tnfip6–/– COCs can be restored by supplementing recombinant TNFIP6 in the culture medium. Scale bars: 100 µm.