Fig. 6. Maintenance of a basal process during RNEC division. (A-D) Snapshots of a time-lapse video recording showing a GFP⁺ RNEC that maintained its basal process (arrows) during division. This cell divided with a horizontal spindle. The cleavage plane is indicated with arrowheads in D. The basal process seems to be asymmetrically inherited by the daughter cell on the left. (E-G) Confocal micrographs of a different GFP⁺ RNEC in metaphase, labeled with propidium iodide (PI). The PI label is shown in E, GFP in F and a merged image in G. The arrow in F points to the metaphase plate and the arrowhead in G points to the basal process.