Fig. 8. Decreased Bmp4 signaling in p107/p130 knockout mice epidermis. (A) Northern analysis of different signaling molecules involved in hair development. Total RNA from skin samples of the quoted genotypes were probed for the expression of Axin, ß-catenin, Lef1, Shh, Waf/Cip1 and Bmp4. The membranes were also hybridized with a 7S RNA probe to verify that equal amounts of mRNA were loaded and transferred. Note that a significant decrease in Bmp4 was the only observed alteration in doubly deficient mice samples. (B-H) Immunohistochemical detection of Bmp4 (B,B',F), p75NTR (C,C'), noggin (D,D',G) and Hgf (E,E',H) in hair follicles from control (B-E), p107/p130-deficient skin (B'-E') and in transplants from p107/p130-deficient skin onto NOD/scid mice (F-H). Note the altered expression of Bmp4, noggin and Hgf that are restored in grafts. Scale bars: 25 µm in E'; 500 µm in H.