Fig. 3. Ring gland defects in Fas2^spin. (A) Third instar larval brain and ring gland (outlined) stained using the Kurs21-GAL4; UAS-synaptobrevinGFP (green) line and anti-Fas2 (red). nCA, neurons innervating the corpora allata and expressing GAL4. (B) The ring gland with its three different regions (corpora cardiaca, corpora allata and prothoracic gland). The axon processes of the neurosecretory cells controlling juvenile hormone synthesis are shown in yellow. These neurons express Fas2 in the axon processes and project into the corpora allata. (C,D) Anti-Fas2 staining of a wild-type (C) or spin (D) ring gland (green). Phalloidin-TRITC is in red. (E,F) Close-up of the corpora allata synapse from a wild-type (E) or Fas2^spin (F) ring gland.