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Fig. 4. Expression of Fas2 in neurons innervating the corpora allata rescues Fas2spin rotation defects. (A) Summary of rescue experiments using lines expressing GAL4 in different subsets of neurons in the brain. Rescued males had the following genotype: Fas2spinR5/Y; UAS-Fas2; GAL4. +++, full rescue; +, partial rescue; –, no rescue. (B) Ring gland expressing a synaptobrevin-GFP fusion protein (green; to mark terminal boutons) in neurons innervating the corpora allata (nCA), using the Kurs21-GAL4 line. The ring gland (outlined) is double stained with anti-Fas2 (red). (C) Ring gland expressing EGFP (green) in neurons innervating the prothoracic gland (nPT), using the Feb211-GAL4 line. The ring gland (outlined) is double stained with anti-Fas2 (red).