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Fig. 2. Effects of overexpression of Cyr61 and similarity with the phenotype obtained following injection of antisense morpholino oligonucleotides. (A-C) Control stage 21 (A), Xcyr61-(B) and MO1-injected (C) embryos. Note the disruption of gastrulation. (D-F) Intra-blastocoelic injections of BSA (D) and mouse Cyr61 (E,F). Note the similarity of the phenotypes in E,F to those in B,C. (G-I) Analysis of isolated dorsal marginal zone regions highlights defects in epiboly caused by overexpression (500 pg RNA; H) or downregulation (I) of Xcyr61. Note that the endoderm in H and I is not covered by epidermis (arrows). Scale bar in A: ~0.4 mm for A-I.