Fig. 5. A model for the lateral localization of lch5 organs. Anterior is towards the left and dorsal is upwards in all panels. lch5 organs in embryonic stages 12-16. The neurons are depicted in red, ligament cells in light green, scolopale cells in light blue, cap cells in dark green and the attachment cells in dark blue. In early stage 12 the ligament cells are located most dorsally within the developing organ. During stage 12 and early stage 13, they migrate ventrally, pulling with them the neuron, scolopale and cap cells. This leads to the rotation of the whole organ. The attachment cells do not change their position. Once the organs acquire the right orientation the ligament cells stretch ventrally and attach to the ectoderm in the lateral region. For simplicity, the lch5 cells in stages 12-13 are drawn next to each other, whereas in reality they are more tightly packed. (B) An embryo overexpressing abd-A under the regulation of da-Gal4, stained with anti 85E. dch3 organs in T2 and T3 segments have descended and reversed their orientation (compare with dch3 in figure 1F). Arrows and arrowheads indicate ligament and cap cells, respectively. (C) A stage 12 wild-type embryo stained with anti-VVL. Note the stronger VVL expression in the dorsal half of the embryo.