Fig. 3. Neuronal patterning is affected in pac mutants. (A-D) Two dpf wild-type (A), pac^rw95 (B), pac^rw95/fr7 (C) and pac^fr7 (D) retinae labeled with zn5 antibody (red), which stains axons and cytoplasm of RGCs. Nuclei of retinal cells are counter-stained green with Sytox Green Nucleic Acid Stain. RGCs differentiate in all mutants (white arrowheads) but their localization is progressively more disrupted in pac^rw95, pac^rw95/fr7 and pac^fr7 eyes. (E-H) Four dpf wild-type (E), pac^rw95 (F), pac^rw95/fr7 (G) and pac^fr7 (H) retinae labeled with anti-Pax6 antibody, which stains amacrine cells (red). (I-L) Four dpf wild-type (I), pac^rw95 (J), pac^rw95/fr7 (K) and pac^fr7 (L) retinae labeled with zpr-1 antibody, which stains double-cone photoreceptors (red). In pac^rw95, the photoreceptor cell layer is normal. In pac^rw95/fr7, the photoreceptor cell layer is locally disrupted and in the pac^fr7 retina, zpr-1-positive photoreceptors do not form an outer layer, but instead are located sparsely near the scleral region of the eye (white arrowheads). a, amacrine cell layer; pl, photoreceptor layer; rgl, retinal ganglion cell layer.