Fig. 2. Upregulation of Oct3/4 expression in ES cells induces neural marker gene expression under serum-free LIF-deficient conditions. (A) ZHTc6 cells were cultured for 8 days in the presence or absence of Tc. Total RNA was extracted from each culture and subjected to RT-PCR analysis for the genes indicated on the right. (B) RNA was extracted from each ZHTc6 cell culture on days 2, 4 and 8, and subjected to RT-PCR analysis for Fgf5 expression.